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Over a wide selection of wavelengths from 800 to 260 nm. The instrument time resolution is about 250 fs and all of the experiments were performed at the magic angle (54.7. Samples were kept stirring for the duration of irradiation to prevent heating and photobleaching. Experiments with the neutral FAD and FADHstates had been carried out beneath aerobic conditions, whereas those with all the anionic FADand FADHstates have been executed beneath anaerobic circumstances. All experiments have been performed in quartz cuvettes with a 5-mm optical length except that the FADHexperiments probed at 270 and 269 nm have been carried out in quartz cuvettes with a 1-mm optical length. ACKNOWLEDGMENTS. This work is supported in element by National Institutes of Health Grants GM074813 and GM31082, the Camille Dreyfus TeacherScholar (to D.Z.), the American Heart Association fellowship (to Z.L.), plus the Ohio State University Pelotonia fellowship (to C.T. and J.L.).18. Byrdin M, Eker APM, Vos MH, Brettel K (2003) Dissection on the triple tryptophan electron transfer chain in Escherichia coli DNA photolyase: Trp382 would be the main donor in photoactivation. Proc Natl Acad Sci USA 100(15):8676681. 19. Kao Y-T, et al. (2008) Ultrafast dynamics of flavins in 5 redox states. J Am Chem Soc 130(39):131323139. 20. Seidel CAM, Schulz A, Sauer MHM (1996) Nucleobase-specific quenching of fluorescent dyes. 1. Nucleobase one-electron redox potentials and their correlation with static and dynamic quenching efficiencies. J Phys Chem one hundred(13):5541553. 21. Gindt YM, Schelvis JPM, Thoren KL, Huang TH (2005) Substrate binding modulates the reduction prospective of DNA photolyase. J Am Chem Soc 127(30):104720473. 22. Vicic DA, et al. (2000) Oxidative repair of a thymine dimer in DNA from a distance by a covalently linked organic intercalator. J Am Chem Soc 122(36):8603611. 23. Byrdin M, et al. (2010) Quantum yield measurements of short-lived photoactivation intermediates in DNA photolyase: Toward a detailed understanding of your triple tryptophan electron transfer chain. J Phys Chem A 114(9):3207214. 24. Saxena C, Sancar A, Zhong D (2004) Femtosecond dynamics of DNA photolyase: Power transfer of antenna initiation and electron transfer of cofactor reduction. J Phys Chem B 108(46):180268033. 25. Park HW, Kim ST, Sancar A, Deisenhofer J (1995) Crystal structure of DNA photolyase from Escherichia coli. Science 268(5219):1866872. 26. Zoltowski BD, et al. (2011) Structure of full-length Drosophila cryptochrome. Nature 480(7377):39699. 27.Migalastat Epigenetics Balland V, Byrdin M, Eker APM, Ahmad M, Brettel K (2009) What makes the distinction among a cryptochrome and DNA photolyase A spectroelectrochemical comparison on the flavin redox transitions.Azemiglitazone Autophagy J Am Chem Soc 131(two):42627.PMID:24423657 28. Partch CL, Clarkson MW, Ozg S, Lee AL, Sancar A (2005) Function of structural plasticity in signal transduction by the cryptochrome blue-light photoreceptor. Biochemistry 44(10):3795805. 29. Antony J, Medvedev DM, Stuchebrukhov AA (2000) Theoretical study of electron transfer in between the photolyase catalytic cofactor FADH- and DNA thymine dimer. J Am Chem Soc 122(six):1057065. 30. Web page CC, Moser CC, Chen XX, Dutton PL (1999) Natural engineering principles of electron tunnelling in biological oxidation-reduction. Nature 402(6757):472. 31. Maul MJ, et al. (2008) Crystal structure and mechanism of a DNA (6-4) photolyase. Angew Chem Int Ed Engl 47(52):100760080. 32. Li J, Uchida T, Todo T, Kitagawa T (2006) Similarities and variations between cyclobutane pyrimidine dimer photolyase and (six.

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