Ration of the antibody to BGLF5.Author ContributionsConceived and developed the experiments: RP GM LH AEG SB JS. Performed the experiments: RP AEG LH SB. Analyzed the data: RP KPY AEG LH SB JS GM MN. Contributed reagents/materials/analysis tools: SFL HJD. Wrote the paper: RP GM JS.scent protein synthesis on a international scale; point mutations in the simple region impair ZEBRA’s host shutoff activity. 293 cells were transfected with pHD1013, or vectors
Ling et al. BMC Genomics 2014, 15:624 http://www.biomedcentral/1471-2164/15/RESEARCH ARTICLEOpen AccessFunctional transcriptome analysis with the postnatal brain from the Ts1Cje mouse model for Down syndrome reveals international disruption of interferon-related molecular networksKing-Hwa Ling1,2,3*, Chelsee A Hewitt2,four, Kai-Leng Tan1,5, Pike-See Cheah1,five, Sharmili Vidyadaran1,six, Mei-I Lai1,6, Han-Chung Lee1, Ken Simpson2, Lavinia Hyde2, Melanie A Pritchard7, Gordon K Smyth2, Tim Thomas2 and Hamish S Scott2,eight,9*AbstractBackground: The Ts1Cje mouse model of Down syndrome (DS) has partial triplication of mouse chromosome 16 (MMU16), which is partially homologous to human chromosome 21. These mice create various neuropathological functions identified in DS individuals. We analysed the impact of partial triplication with the MMU16 segment on international gene expression within the cerebral cortex, cerebellum and hippocampus of Ts1Cje mice at four time-points: postnatal day (P)1, P15, P30 and P84. Outcomes: Gene expression profiling identified a total of 317 differentially expressed genes (DEGs), chosen from various spatiotemporal comparisons, in between Ts1Cje and disomic mice. A total of 201 DEGs had been identified in the cerebellum, 129 from the hippocampus and 40 from the cerebral cortex. Of these, only 18 DEGs had been identified as prevalent to all three brain regions and 15 were situated in the triplicated segment. We validated eight chosen DEGs in the cerebral cortex (Brwd1, Donson, Erdr1, Ifnar1, Itgb8, Itsn1, Mrps6 and Tmem50b), 18 DEGs in the cerebellum (Atp5o, Brwd1, Donson, Dopey2, Erdr1, Hmgn1, Ifnar1, Ifnar2, Ifngr2, Itgb8, Itsn1, Mrps6, Paxbp1, Son, Stat1, Tbata, Tmem50b and Wrb) and 11 DEGs in the hippocampus (Atp5o, Brwd1, Cbr1, Donson, Erdr1, Itgb8, Itsn1, Morc3, Son, Tmem50b and Wrb). Functional clustering analysis on the 317 DEGs identified interferon-related signal transduction as the most drastically dysregulated pathway in Ts1Cje postnatal brain development. RT-qPCR and western blotting evaluation showed both Ifnar1 and Stat1 had been over-expressed in P84 Ts1Cje cerebral cortex and cerebellum as in comparison with wild variety littermates. Conclusions: These findings recommend over-expression of interferon receptor may possibly bring about over-stimulation of Jak-Stat signaling pathway which may contribute towards the neuropathology in Ts1Cje or DS brain.Quassin Formula The part of interferon mediated activation or inhibition of signal transduction including Jak-Stat signaling pathway has been well characterized in several biological processes and illness models which includes DS but data pertaining to the part of this pathway within the improvement and function of the Ts1Cje or DS brain remains scarce and warrants further investigation.Anti-Mouse H-2K Antibody Protocol * Correspondence: lkh@upm.PMID:23522542 edu.my; [email protected] Equal contributors 1 Genetics and Regenerative Medicine Study Centre, Faculty of Medicine and Wellness Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia 2 Walter and Eliza Hall Institute of Healthcare Investigation, 1G Royal Parade, Parkville, V.
