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nutrientsArticlePotentially Bio-Accessible Metabolites from an Extract of Cornus mas Fruit following Gastrointestinal Digestion In Vitro and Gut Microbiota Ex Vivo TreatmentAgata Ol dzka 1, , Katarzyna Cichocka 1, , Konrad Wolinski 2 , Matthias F. Melzig 3 e and Monika E. Czerwinska four,5, Student Scientific Association “Farmakon”, Division of Biochemistry and Pharmacogenomics, Health-related University of Warsaw, 1 Banacha Street, 02-097 Warsaw, Poland; agata.bobinska2997@gmail (A.O.); katarzyna.cichocka.kc@gmail (K.C.) Polish Academy of Sciences Botanical Garden, Centre for Biological Diversity Conservation in Powsin, two Prawdziwka Street, 02-973 Warsaw, Poland; [email protected] Institute of Pharmacy, Freie Universitaet Berlin, 2+4 Koenigin-Luise Street, 14195 Berlin, Germany; [email protected] Division of Biochemistry and Pharmacogenomics, Faculty of Pharmacy, Health-related University of Warsaw, 1 Banacha Street, 02-097 Warsaw, Poland Centre for Preclinical Study, Healthcare University of Warsaw, 1B Banacha Street, 02-097 Warsaw, Poland Correspondence: [email protected]; Tel.: +48-22-116-61-85 These authors contributed equally to this function.Citation: Ol dzka, A.; Cichocka, K.Humulone Formula ; e Wolinski, K.6-Methoxydihydrosanguinarine Formula ; Melzig, M.PMID:23381626 F.; Czerwinska, M.E. Potentially Bio-Accessible Metabolites from an Extract of Cornus mas Fruit just after Gastrointestinal Digestion In Vitro and Gut Microbiota Ex Vivo Remedy. Nutrients 2022, 14, 2287. doi.org/10.3390/ nu14112287 Academic Editor: Karolina Szewczyk-Golec Received: 28 April 2022 Accepted: 26 May 2022 Published: 30 May possibly 2022 Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.Abstract: Targeting pancreatic lipase and -amylase by digestion-derived fractions of ethanolicaqueous (60 , v/v) extract from Cornus mas fruit (CM) in relation towards the handle and prevention of metabolic disorders, such as diabetes, was the initial purpose with the present study. Taking into consideration the significance of bio-accessibility of compounds, we attempted to identify metabolites of CM immediately after gastrointestinal digestion in vitro, also as their kinetic changes upon gut microbiota therapy. The digestion of extract was simulated with digestive enzymes in vitro and human gut microbiota ex vivo (1 h, 3 h, 6 h, 24 h), followed by chromatographic evaluation employing the UHPLC-DAD-MSn technique. The effect of fractions from gastrointestinal digestion in vitro around the activity of pancreatic lipase and -amylase was studied with fluorescence-based assays. The.
