Was made as much as the mark with the mobile phase to acquire a remedy containing 30 /ml of DIC. This remedy was applied for the estimation of DIC. The solution is further diluted with mobile phase to acquire 2.five /ml MEF and 5 /ml of PCM, respectively. Both the options had been sonicated for 10 min. Solutions have been injected as per the above chromatographic situations and peak places were recorded. The quantifications were carried out by keeping these values to the straight line equation of calibration curve.Outcomes AND DISCUSSIONThe objective of the method development was to resolve chromatographic peaks for active drug components with less asymmetric factor. The mobile phase acetonitrile:20 mM potassium dihydrogen phosphate (70:30 v/v) adjusted to pH 4 utilizing orthophosphoric acid was discovered to be satisfactory which gave 3 symmetric and wellresolved peaks for DIC, MEF and PCM. The retention times of DIC, MEF and PCM have been three.8, 9.3 and two.5 min, respectively (fig. 1). The resolution between DIC, MEF and PCM was discovered to become much more than 2, which indicates good separation of all the compounds. The asymmetric variables for DIC, MEF and PCM have been 1.36, 1.14, 1.44, respectively. The mobile phase flow price was maintained at 1 ml/min. Overlaid UV spectra of each the drugs showed that DIC, MEF and PCM absorbed appreciably at 220 nm, so detection was carried out at 220 nm.Fig. 1: Chromatogram of typical PCM, DIC and MEF. Chromatogram of normal options of paracetamol (PCM, 2.five min) dicyclomine (DIC, 3.8 min) and mefenamic acid (MEF, 9.3 min) obtained in mobile phase. November – December 2014 Indian Journal of Pharmaceutical SciencesijpsonlineLinearity was evaluated by analysis of operating typical options of DIC, MEF and PCM of 5 distinctive concentrations along with the method was discovered to be linear inside the array of ten?00 /ml for DIC, 0.05?0 /ml for MEF and 0.1?0 /ml for PCM, respectively. The regression data obtained are represented in Table 1. Instrument precision was determined by performing injection repeatability test and the relative common deviation values for DIC, MEF and PCM have been identified. The intraday and interday precision research were carried out for three concentrations of DIC, MEF and PCM and the final results are reported in Table two. The accuracy on the strategy was determined by calculating recoveries of DIC, MEF and PCM by strategy of regular addition. Recoveries were discovered to become 97.83?9.26, 98.98?9.53 and 99.79?00.16 for DIC, MEF and PCM, respectively (Table 2). Recovery studies had been performed in triplicate. The LOQ for DIC, MEF and PCM had been identified to be ten, 0.05 and 0.1 /ml respectively. The LOD for DIC, MEF and PCM had been found to become three, 0.0125 and 0.033 /ml respectively (Table 2). Robustness study was performed by deliberately altering the experimental circumstances like flow rate from 1 ml/min to 0.eight ml/min and 1.two ml/min. The composition of mobile phase was changed varying the JAK2 Inhibitor custom synthesis proportion of CA XII Inhibitor MedChemExpress acetonitrile by five . In each the circumstances the recovery of all the drugs had been determined and the RSD was discovered to become less than two . Answer stability of DIC, MEF and PCM had been evaluated at area temperature for 24 h. All of the drugs had been discovered to become steady with a recovery of far more than 98 . System suitability parameters like the number of theoretical plates, resolution, and peak assymetry had been determined and reported in Table 2. The proposed system was successfully applied to the determination of DIC, MEF and PCM in their combined dosage form. The recovery was.