ly different in between the high-yielding strain (H) and the low-yielding strain (L) in the 3 modules of blue, brown, and bisque4. The results of correlation analysis in between two modules (Supplementary Figure S4) show that blue and brown, and blue and bisque4 are substantially negatively correlated, with correlation coefficients of – 0.7 and – 0.59, respectively. Brown and bisque4 are weakly correlated, having a correlation coefficient of 0.24. GO and KEGG enrichment analysis on blue, brown, and bisque4.GO enrichment analysis was carried out on genes inside the three modules of blue, brown, and bisque4, respectively (Supplementary Figure S5). The outcomes showed that genes in these three modules were primarily enriched in catalytic activity and binding inside the molecular functions; metabolic processes, cellular processes, and single-organism processes inside the biological processes; and cell and cell parts within the cellular component. The 3 modules had the same GO enrichment outcomes, only the amount of genes was various. Furthermore, KEGG enrichment PARP2 web benefits (Supplementary Table S3) for the 3 modules have been not precisely the same. The brown module (P 0.05) was mostly enriched inside the metabolic pathways of glyceride, sulfur, and galactose; non-homologous end joining; and microbial metabolism in 5-HT6 Receptor Modulator Compound diverse environments (Fig. 3). The blue module (P 0.05) was primarily enriched in the metabolism and biosynthesis of numerous amino acids; metabolism of oxycarboxylic acid, and folate; biosynthesis of secondary metabolites, aminoacyl-tRNA, pantothenate, and CoA; microbial metabolism in diverse environments; basalResultsScientific Reports | Vol:.(1234567890)(2021) 11:18207 |doi.org/10.1038/s41598-021-97616-nature/scientificreports/Figure 1. Venn diagrams of DEGs. (a) Venn diagram of DEGs throughout 3 cultivation periods of high-yielding strains; (b) Venn diagram of DEGs for the duration of 3 cultivation periods on the low-yielding strains; (c) Venn diagram of DEGs between high-yielding and low-yielding strains in three culture periods; (d) Venn diagram of DEGs in high-yielding strains, low-yielding strains, in between high- and low-yielding strains. transcription things, and so on. (Fig. four). The bisque4 module (P 0.05) was mainly enriched within the cell cycle; meiosis; DNA repair; mismatch repair; nucleotide excision repair; base excision repair; biosynthesis of terpenoid backbones, and unsaturated fatty acids; and fatty acid metabolism (Fig. five). The KEGG enrichment benefits of the 3 modules have been substantially various, which was constant with the results with the module correlation analysis. The genes associated with triterpenoid anabolism in every module had been selected in accordance with KEGG annotation final results of genes, and these genes together with the above gene’s expression correlation weight worth among inside the module were top 10 were chosen (Supplementary Table S4). These genes were selected for GO and KEGG enrichment. GO enrichment (Supplementary Figure S6) showed that these selected genes had been primarily concentrated in catalytic activity and binding in the molecular functions; metabolic processes, cellular processes, and single-organism processes in the biological processes; and cell and cell components in the cellular component. The enrichment of these three modules’ genes was nevertheless basically the same. Detailed GO information and facts of these 3 modules’ genes is displayed in Supplementary Tables S5. KEGG enrichment final results of genes associated with triterpenoid biosynthesis in every module (Supplementary Table S8
