ly distinctive in between the high-yielding strain (H) as well as the low-yielding strain (L) inside the three modules of blue, brown, and bisque4. The outcomes of correlation evaluation between two modules (Supplementary Figure S4) show that blue and brown, and blue and bisque4 are substantially negatively correlated, with correlation coefficients of – 0.7 and – 0.59, respectively. Brown and bisque4 are weakly correlated, having a correlation coefficient of 0.24. GO and KEGG enrichment analysis on blue, brown, and bisque4.GO enrichment evaluation was carried out on genes in the 3 modules of blue, brown, and bisque4, respectively (Supplementary Figure S5). The outcomes showed that genes in these three modules have been mainly enriched in catalytic activity and binding inside the molecular functions; metabolic processes, mGluR Formulation cellular processes, and RSK2 manufacturer single-organism processes in the biological processes; and cell and cell parts inside the cellular component. The 3 modules had the identical GO enrichment outcomes, only the amount of genes was distinctive. Furthermore, KEGG enrichment final results (Supplementary Table S3) for the three modules had been not the exact same. The brown module (P 0.05) was mostly enriched within the metabolic pathways of glyceride, sulfur, and galactose; non-homologous end joining; and microbial metabolism in diverse environments (Fig. 3). The blue module (P 0.05) was primarily enriched within the metabolism and biosynthesis of several amino acids; metabolism of oxycarboxylic acid, and folate; biosynthesis of secondary metabolites, aminoacyl-tRNA, pantothenate, and CoA; microbial metabolism in diverse environments; basalResultsScientific Reports | Vol:.(1234567890)(2021) 11:18207 |doi.org/10.1038/s41598-021-97616-nature/scientificreports/Figure 1. Venn diagrams of DEGs. (a) Venn diagram of DEGs in the course of three cultivation periods of high-yielding strains; (b) Venn diagram of DEGs throughout three cultivation periods from the low-yielding strains; (c) Venn diagram of DEGs among high-yielding and low-yielding strains in 3 culture periods; (d) Venn diagram of DEGs in high-yielding strains, low-yielding strains, between high- and low-yielding strains. transcription factors, and so forth. (Fig. four). The bisque4 module (P 0.05) was mostly enriched in the cell cycle; meiosis; DNA repair; mismatch repair; nucleotide excision repair; base excision repair; biosynthesis of terpenoid backbones, and unsaturated fatty acids; and fatty acid metabolism (Fig. 5). The KEGG enrichment outcomes from the 3 modules were drastically diverse, which was consistent using the final results from the module correlation analysis. The genes associated with triterpenoid anabolism in every module were selected as outlined by KEGG annotation benefits of genes, and those genes with all the above gene’s expression correlation weight worth among inside the module had been top rated 10 had been selected (Supplementary Table S4). Those genes were chosen for GO and KEGG enrichment. GO enrichment (Supplementary Figure S6) showed that these selected genes have been primarily concentrated in catalytic activity and binding within the molecular functions; metabolic processes, cellular processes, and single-organism processes inside the biological processes; and cell and cell components inside the cellular component. The enrichment of those 3 modules’ genes was nevertheless generally exactly the same. Detailed GO info of those 3 modules’ genes is displayed in Supplementary Tables S5. KEGG enrichment outcomes of genes associated with triterpenoid biosynthesis in every single module (Supplementary Table S8
