Name :
Recombinant Mouse TL1A/TNFSF15 Protein (Trimer, His Tag), HPLC-verified
Biological Activity :
Background :
TL1A, also known as TNFSF15, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is specifically expressed in endothelial cells. TL1A also can be detected in monocytes, placenta, lung, liver, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine and colon. TL1A is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It mediates activation of NF-kappa-B. It also inhibits vascular endothelial growth and angiogenesis (in vitro). TL1A promotes activation of caspases and apoptosis. It is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor.
Biological Activity :
1.Immobilized Recombinant Human DCR3 / TNFRSF6B Protein (Fc Tag) (Cat: 10224-H02B) at 2 μg/mL (100 μl/well) can bind Recombinant Mouse TL1A/TNFSF15 Protein (His Tag) (Cat: 5A7685-M07H1), the EC50 is 8-24 ng/mL.2.Recombinant Mouse TL1A/TNFSF15 Protein, His Tag, HPLC-verified（Cat. No. 5A7685-M07H1）was more than 90% and the molecular weight of this protein is around 73.1 kDa verified by SEC-MALS（Routinely tested）.
Expression Host :
Mouse
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NCBI Gene ID :
Synonyms :
Synonyms :
tumor necrosis factor (ligand) superfamily, member 15
Amino Acid Sequence :
Molecular Weight :
The recombinant Mouse TNFSF15 has a calculated molecular mass of 62.01 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 72.4 kDa protein in SDS-PAGE under reducing conditions.
Purity :
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the Mouse TNFSF15 (Q5UBV8) (Ile76-Leu252) was expressed, with a polyhistidine tag at the N-terminus. This construct has a trimer design.
Buffer Solution :
Lyophilized from sterile PBS, 5% Trehalose, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
Tl1 Protein, Mouse; TL1A Protein, Mouse; Vegi Protein, Mouse TL1A/TNFSF15 背景信息 TL1A, also known as TNFSF15, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is specifically expressed in endothelial cells. TL1A also can be detected in monocytes, placenta, lung, liver, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine and colon. TL1A is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It mediates activation of NF-kappa-B. It also inhibits vascular endothelial growth and angiogenesis (in vitro). TL1A promotes activation of caspases and apoptosis. It is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor.
References & Citations :
Jin T, et al. (2007) X-ray crystal structure of TNF ligand family member TL1A at 2.1A. Biochem Biophys Res Commun. 364(1):1-6.Cassatella MA, et al. (2007) Soluble TNF-like cytokine (TL1A) production by immune complexes stimulated monocytes in rheumatoid arthritis. J Immunol. 178(11):7325-33Prehn JL, et al. (2007) The T cell costimulator TL1A is induced by FcgammaR signaling in human monocytes and dendritic cells. J Immunol. 178(7):4033-8.
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