Biotinylated Interleukin 8 (IL-8 /CXCL8) 8024

Additional Information:
accession P10145|express systemE.coli|product tagbiotin at C-terminal|purity> 97% by SDS PAGE|molecular weightPredicted Molecular Mass: 10,803.49 Da Extinction Coefficient: 12,900 M-1 cm-1 Actual Molecular Mass: 10,803 Da by ESI Mass Spec|available size10 g, 100 g, 2 g, 50 g|endotoxinBiotinylated Interleukin 8 (IL-8 /CXCL8) 8024proteinDatabase link:human P10145 Size and concentration2, 10, 50, 100g and lyophilizedFormLyophilizedStorage InstructionsAvoid repeated freeze-thaw cycles: 12 months from date of receipt, -20 to -70 C as supplied. 1 month, 2 to 8 C under sterile conditions after reconstitution. 3 months, -20 to -70 C under sterile conditions after reconstitutionStorage bufferReconstitution: Spin sample prior to reconstitution. Recommended concentration of 100g/mL in sterile water.Shipping: Room TempPurity> 97% by SDS PAGE and HPLCtarget relevanceInterleukin 8 (IL-8 )(CXCL8) is secreted primarily by macrophages and monocytes. It is one of the key mediators for inflammatory responses. IL-8 is a strong chemotractant for neutrophils and monoccytes, and promotes activation of these target cells by binding to two cell surface receptors CXCR1 and CXCR2. It is also a strong angiogenic agent, and is considered to play a role in the pathogenesis of bronchiolitis.Biotinylated CXCL8 is made using the enzymatic method, which has several advantages over chemical biotinylation methods. The attachment of biotin at a specific lysine residue is nearly 100% complete, and leads to a modified chemokine with functionalities comparable to those of the unmodified CXCL8 in migration assay. Utilizing avidin/streptavidin analogues conjugated to various fluorescent labels, biotinylated CXCL8 is useful in studies on receptor identification, distribution, chemokine binding, and other cellular assays. They serve as great tools in visualization and quantification, and can replace radioactively labeled chemokines.Protein namesInterleukin-8 (IL-8) (C-X-C motif chemokine 8) (Chemokine (C-X-C motif) ligand 8) (Emoctakin) (Granulocyte chemotactic protein 1) (GCP-1) (Monocyte-derived neutrophil chemotactic factor) (MDNCF) (Monocyte-derived neutrophil-activating peptide) (MONAP) (Neutrophil-activating protein 1) (NAP-1) (Protein 3-10C) (T-cell chemotactic factor) [CGene namesCXCL8,CXCL8 IL8Protein familyIntercrine alpha (chemokine CxC) familyMass11098DaFunctionChemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed:18692776, PubMed:7636208). Also plays an important role in neutrophil activation (PubMed:2145175, PubMed:9623510). Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells (PubMed:1840701, PubMed:1891716). G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways (PubMed:11971003, PubMed:8662698).Subellular locationSecreted.StructureHomodimer (PubMed:31235521). Dimer formation is disrupted by tick evasin-3 (PubMed:31235521). Interacts with TNFAIP6 (via Link domain); this interaction interferes with chemokine binding to glycosaminoglycans.Post-translational modificationSeveral N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.; Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation.; (Microbial infection) Cleaved by group A Streptococcus protease SpyCEP; leading to impaired neutrophil endothelial transmigration and thus increased virulence.Target Relevance information above includes information from UniProt accession: P10145The UniProt Consortium|