Ymer, Video S2: Flammability test for CNF composite (30 vol ), Video S
Ymer, Video S2: Flammability test for CNF composite (30 vol ), Video S3: Flammability test for CNF composite (55 vol ), Video S4: More flammability test for CNF composite (80 vol ). Author Contributions: Conceptualization, T.S., L.A.B. and W.S.; experimental design, investigation, W.S. W.S. mostly wrote the Methyl jasmonate custom synthesis manuscript with contributions from S.F., L.A.B. and T.S. All authors have read and agreed towards the published version on the manuscript. Funding: This research was funded by JST-Mirai R D System (JPMJMI17ED), JSPS Grant-inAids for Scientific Investigation (A) (21H04733), and JSPS Grant-in-Aids for Young Scientists (19J22463, 20K15567). LB is supported by the European Study Council (ERC), grant agreement No. 742733. Information Availability Statement: The information that support the findings of this study are available in the corresponding authors upon reasonable request. Acknowledgments: TEMPO-oxidized pulp was kindly supplied by DKS Co. Ltd. Conflicts of Interest: The authors declare no conflict of interest.
nanomaterialsArticleQuantitative Estimation of Cell-Associated Silver Nanoparticles Employing the Normalized Side Scattering Intensities of Flow CytometryYoo Jin Shim 1, , My Kieu Ha 1,, and Tae Hyun Yoon 1,2,3, 2Department of Diversity Library Advantages Chemistry, College of All-natural Sciences, Hanyang University, Seoul 04763, Korea; [email protected] (Y.J.S.); [email protected] (M.K.H.) Institute of Next Generation Material Style, Hanyang University, Seoul 04763, Korea Research Institute for Convergence of Fundamental Science, Hanyang University, Seoul 04763, Korea Correspondence: [email protected]; Tel.: 82-2-2220-4593 These authors contributed equally to this operate. Current Address: Vaccine Infectious Illness Institute, University of Antwerp, 2610 Antwerpen, Belgium.Citation: Shim, Y.J.; Ha, M.K.; Yoon, T.H. Quantitative Estimation of Cell-Associated Silver Nanoparticles using the Normalized Side Scattering Intensities of Flow Cytometry. Nanomaterials 2021, 11, 3079. https:// doi.org/10.3390/nanoAbstract: Quantification of cellular nanoparticles (NPs) is amongst the most important methods in studying NP ell interactions. Here, a simple technique for the estimation of cell-associated silver (Ag) NPs in lung cancer cells (A549) is proposed according to their side scattering (SSC) intensities measured by flow cytometry (FCM). To estimate cellular Ag NPs related with A549 cells more than a broad array of experimental conditions, we measured the normalized SSC intensities (nSSC) of A549 cells treated with Ag NPs with five various core sizes (i.e., 4000 nm, positively charged) below many exposure situations that reflect distinct circumstances of agglomeration, diffusion, and sedimentation in cell culture media, such as upright and inverted configurations with various media heights. Then, we correlated these nSSC values with all the numbers of cellular Ag NPs determined by inductively coupled plasma mass spectrometry (ICPMS) as a well-established cross-validation system. The diverse core sizes of Ag NPs and the various exposure conditions tested in this study confirmed that the FCM-SSC intensities are highly correlated with their core sizes at the same time because the amount of cellular Ag NPs over a linear variety as much as 80,000 Ag NPs/cell and 23 nSSC, which is significantly broader than those of preceding studies. Keyword phrases: cellular association of nanoparticles; flow cytometry; silver nanoparticlesAcademic Editor: Vincenzo Amendola Received: 15 October 2021 Accepted: ten November 2021 Published:.
